Dietary cadmium and benzo(a)pyrene increased intestinal metallothionein expression in the fish Fundulus heteroclitus

Dietary cadmium and benzo(a)pyrene increased intestinal metallothionein expression in the fish Fundulus heteroclitus - Alejandro Perez Matus

Research about Dietary cadmium and benzo(a)pyrene increased intestinal metallothionein expression in the fish Fundulus heteroclitus

Fish were individually fed food pellets containing cadmium, benzo(a)pyrene, or a combination of the two, then analyzed for metallothionein mRNA expression in the intestine, liver, and gill using real-time RTqPCR. An initial experiment using only cadmium showed that ingestion of pellets varied in individual fish, and estimates of cadmium dose from the numbers of ingested pellets indicated considerable individual variability in cadmium dose. Induction of intestinal metallothionein mRNA was apparent, however, and a linear dose–response relationship was observed for metallothionein expression and cadmium dose in the intestine, but not the other organs, which showed no induction. In a second experiment, the ntire daily cadmium dose was provided in a single contaminated pellet that was consumed by all treated fish, effectively eliminating the effect of variable ingestion rates on dose, and the interaction between cadmium and benzo(a)pyrene was also investigated. The intestine was again the primary organ for metallothionein induction by cadmium. When benzo(a)pyrene was administered together with cadmium, induction of metallothionein was potentiated by the presence of benzo(a)pyrene, with the main effect seen in the intestine, where already high levels of induction by cadmium alone increased by 1.74-fold when benzo(a)pyrene was present.

Descargar y citar documento completo
Descargar DocumentoG. Roesijadi, S. Rezvankhah, A. Perez-Matus, A. Mitelberg, K. Torruellas & P.A. Van VeldDietary cadmium and benzo(a)pyrene increased intestinal metallothione in expression in the fish Fundulus heteroclitus
Related projects
Temperate rocky subtidal reef community reveals human impacts across the entire food web

Alejandro Pérez-Matus Andres Ospina-Alvarez Patricio A. Camus, Sergio A. Carrasco Miriam Fernandez Stefan Gelcich Natalio Godoy F.

Continue reading

Specimen collection: An essential tool

COLLECTING BIOLOGICAL specimens for scientific studies came under scrutiny when B. A. Minteer et al. [“Avoiding (re)extinction,” Perspectives, 18

Continue reading

Nest building and description of parental care behavior in a temperate reef fish, Chromis creams (Pisces: Pomacentridae)

Background: Subtle ecological and behavioral mechanisms that enhance reproduction such as nest building by animals may provide useful information

Continue reading


Global regime shift dynamics of catastrophic sea urchin overgrazing

A pronounced, widespread and persistent regime shift among marine ecosystems is observable on temperate rocky reefs as a result of sea urchin

Continue reading

Length-weight relationships for 25 kelp forest-associated fishes of northern and central Chile

Descargar y citar documento completo Length-weight relationships for 25 kelp forest-associated fishes of northern and central Chile

Continue reading

The early life history of two sympatric New Zealand octopuses: eggs and paralarvae of Octopus huttoni and Pinnoctopus cordiformis

Desc

Continue reading